![]() This way the background created stays undistorted in relation to the original data. If the image is a scaled 3D stack (with indicated units such as µm), the filter will also consider the correct x/y/z scaling ratio and perform the blurring accordingly in 3D space. The pseudo flat field correction takes a copy of the original image to be corrected and blurs it with a Gaussian Blur filter using the specified radius. The Output image is always 32-bit to account for correct float-point values after image division. RGB images are not yet supported (will be available in the future as well). Otherwise, if they are not single slices, dark-field and flat-field need to have the same dimensions as the image to be corrected. Those will be adapted to the original stack slice number. If the original image which sould be corrected for uneven illumination is a stack, flat-field as well as dark-field images can also be provided as single images. The dark-field image can also be ommited if unavailable. The flat field correction allows to correct for uneven illumination including a dark-field (dark current) image subtraction. The following update sites need currently to be minimally active to be able to use all functionalities of the BioVoxxel 3D Boxįunctionalities Filtering Flat Field Correction The BioVoxxel 3D Box are distributed via the BioVoxxel 3D Box update site in Fiji. Also label images created via other tools such as MorphoLibJ are suitable inputs for any plugin using labels. Those labels can be equivalently used like ROIs with many CLIJ2 functions. Segmentation output is based stronger on labels (intensity coding of objects) instead of ROIs. ![]() All functions are heavily based on GPU computing via the fabulous CLIJ2 library. Most of the known BioVoxxel Toolbox functions now for 2D and 3D images in one place. ![]() REMARK: Please inform me about any issues you encounter! View the Project on GitHub biovoxxel/bv3dbox BioVoxxel 3D Box (bv3dbox) The known BioVoxxel Toolbox functions now improved for 2D and 3D images ![]()
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